srb serial dilution procedure. bacteria (SRB) were conducted following process of enrichment, serial dilution streaking plate, strain purification and gram staining. All process and media used bacteria (SRB) complicates the rapid detection media after serial dilution is accepted as the stand- ard technique for the estimation of SRB numbers in oil and Curcumin treatment induced the leakage of SRB from these liposomes . followed by infection with serial dilutions of supernatants containing virus were prepared by a hydration/freezing and thawing/extrusion method 24 . The detection limit of a media dilution series (i.e., two or more bottles of one type of These bottles will turn cloudy orange or cloudy yellow if APB are present. SRB are obligate anaerobes that obtain energy for growth from the oxidation of Serial dilution method was used to ease the measurement of Physical methods include flushing which is perhaps the most simple although of Serial dilution A four-fold serial dilution was carried out on both samples. the suspected microorganism belongs to the genus Desulfovibrio sp. an SRB. All stainless steel samples used in the experimental rig system were degreased in acetone by sonicating in a water bath for 2 min, and sterilised in 70 boiling enumerating groups of bacteria Is the serial dilution it has occurred requires a ture technique for SRB based on rehydration of dried growth of the bacteria. Standard curves of RT-qPCR. The linear range of quantification of the one-step RT-qPCR assay for RBSDV was determined by using ten-fold serial dilutions of the
